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Taq DNA Ligase For Dna Ligation
Taq DNA Ligase For Dna Ligation
Specifications
Brand Name :
GDSBio
Model Number :
E1012
Certification :
/
Place of Origin :
China
MOQ :
1000 U
Price :
USD 30-43/1000 U
Payment Terms :
L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability :
100 Bag/Bags Per Day
Delivery Time :
8 work days
Packaging Details :
small package or bulk distribute or OEM
Product Name :
Taq DNA Ligase
Cat. No./Spec. :
E1012-A/1,000 U; E1012-B/2,000 U; E1012-C/10,000 U
Appearance :
colourless
Application :
DNA ligation
Classification :
General Reagents
Grade :
molecular biology
Logo Printing :
With Logo Printing
Transport Package :
Packing
Production Capacity :
100 Bag/Bags Per Day
Storage Conditions :
Store at -20°C
Description

Taq DNA Ligase

Cat. No./Spec.: E1012-A/1,000 U; E1012-B/2,000 U; E1012-C/10,000 U
Concentration: 40 U/µL

Product Description
Taq DNA Ligase is a heat-resistant ligase that catalyzes the formation of phosphodiester bonds at the 5´ -phosphate and 3´-hydroxyl ends of two adjacent DNA strands. This reaction can occur only when the two oligonucleotide chains are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotide chains. Therefore, it can be used to detect single nucleotide variants. Taq DNA ligase uses NAD as a cofactor. Taq DNA ligase was active in the range of 37-75°C.

Storage Condition & Shelf Life
Store at -20°C. The product is valid for 2 years.

Source
Recombinant E. coli strain containing ligase gene cloned from Thermus aquaticus HB8.

Unit Definition
1 unit is the amount of enzyme required to bind 50% of 1 µg BstEII enzyme to the 12-base pair sticky end of λ DNA for 15 minutes at 45 ° C at a total reaction volume of 50 µl.

Scope of Application
• Using ligase chain reaction (LCR) and ligase detection reaction (LDR) to specifically detect alleles
• Incorporating phosphorylated oligonucleotides during primer extension amplification for mutation detection

Product tags

taq polymerase protocol

,

taq dna polymerase

,

splicing method
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Taq DNA Ligase For Dna Ligation

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Product Detail
Brand Name :
GDSBio
Model Number :
E1012
Certification :
/
Place of Origin :
China
MOQ :
1000 U
Price :
USD 30-43/1000 U
Contact Supplier
Taq DNA Ligase For Dna Ligation

Guangzhou Dongsheng Biotech Co., Ltd

Active Member
4 Years
guangdong, guangzhou
Since 2005
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter
Main Products :
PCR Master Mix, Real Time PCR Mix, One Step RT QPCR Master Mix
Total Annual :
1000000-90000000
Employee Number :
50~100
Certification Level :
Active Member
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