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General Reagent T4 RNA Ligase
General Reagent T4 RNA Ligase
Specifications
Brand Name :
GDSBio
Model Number :
E1015
Certification :
/
Place of Origin :
China
MOQ :
1000 U
Price :
USD 109-121/1000 U
Payment Terms :
L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability :
100 Bag/Bags Per Day
Delivery Time :
8 work days
Packaging Details :
small package or bulk distribute or OEM
Product Name :
T4 RNA Ligase
Cat. No./Spec. :
E1015-A/1,000 U
Appearance :
colourless
Application :
RNA ligation
Classification :
General Reagents
Grade :
molecular biology
Logo Printing :
With Logo Printing
Transport Package :
Packing
Production Capacity :
100 Bag/Bags Per Day
Storage Conditions :
Store at -20°C
Description
T4 RNA Ligase

Cat. No./Spec.: E1015-A/1,000 U
Concentration: 10 U/µL

Product Description
T4 RNA Ligase catalyzes the ATP-dependent formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA, and DNA molecules both intermolecularly and intramolecularly.
Minimum substrates: a nucleotide 3',5'-phosphate in intermolecular reactions and an 8-nucleotide oligonucleotide in intramolecular reactions.
Storage Condition & Shelf Life
Store at -20°C.

Source
Recombinant E. coli strain contains the gene 63 cloned from bacteriophage T4.

Unit Definition
A unit is defined as the amount of enzyme required to catalyze the conversion of 1 nmol of 5'-[32P]-(A)12-18 to its acid-resistant form at 37°C within 30 minutes.
Enzyme activity is determined in the following mixture: 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM DTT, 1 mM ATP, and 10 µM 5'-[32P]-(A)12-18 (10 µM at the 5'-terminus).

Scope of Application
- RNA 3'-terminal labeling (using cytidine 3',5'-di[α-32P] phosphate)
- RNA-to-RNA ligation
- Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
- Specific modification of tRNA
- Ligation of oligodeoxynucleotides to single-stranded cDNA for 5' RACE (Rapid Amplification of cDNA Ends)
- Site-specific generation of PCR primer complexes

Inhibition and Inactivation
- Inhibitors: metal chelating agents, SH group modifying reagents.
- Heat at 70°C for 10 minutes.

This product is for research use only.
Product tags

peptide linkage

,

splicing of ofc

,

oligonucleotide primer
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General Reagent T4 RNA Ligase

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Product Detail
Brand Name :
GDSBio
Model Number :
E1015
Certification :
/
Place of Origin :
China
MOQ :
1000 U
Price :
USD 109-121/1000 U
Contact Supplier
General Reagent T4 RNA Ligase

Guangzhou Dongsheng Biotech Co., Ltd

Active Member
4 Years
guangdong, guangzhou
Since 2005
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter
Main Products :
PCR Master Mix, Real Time PCR Mix, One Step RT QPCR Master Mix
Total Annual :
1000000-90000000
Employee Number :
50~100
Certification Level :
Active Member
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