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DNase I, RNase-Free Molecular Tool Enzyme
DNase I, RNase-Free Molecular Tool Enzyme
Specifications
Brand Name :
GDSBio
Model Number :
E1018
Certification :
/
Place of Origin :
China
MOQ :
1000 U
Price :
USD 76-84.5/1000 U
Payment Terms :
L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability :
100 Bag/Bags Per Day
Delivery Time :
8 work days
Packaging Details :
small package or bulk distribute or OEM
Product Name :
DNase I, RNase-Free, HC
Cat. No./Spec. :
E1018-A/1,000 U
Appearance :
colourless
Application :
cleave both single-stranded and double-stranded DNA
Classification :
General Reagents
Grade :
molecular biology
Logo Printing :
With Logo Printing
Transport Package :
Packing
Production Capacity :
100 Bag/Bags Per Day
Storage Conditions :
Store at -20°C
Description
DNase I, RNase-Free, HC

Cat. No./Spec.: E1018-A/1,000 U
Concentration: 50 U/µL

Product Description
DNase I (without RNase) is an endonuclease that can cleave both single-stranded and double-stranded DNA. It hydrolyzes
phosphodiester bonds, producing single deoxyribonucleotides and oligodeoxyribonucleotides with 5'-phosphate groups and 3'-OH groups.
The activity of this enzyme is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I cuts each strand of dsDNA in a statistically random and independent manner. When Mn2+ is present, the enzyme almost cuts both DNA strands at the same site, resulting in DNA fragments with blunt ends or overhangs of one or a few nucleotides.
Storage Condition & Shelf Life
Store at -20°C.

Source
Recombinant E. coli strain containing the cloned gene encoding bovine DNase I.

Unit Definition
A unit is defined as the amount of enzyme required to completely degrade 1 µg of plasmid DNA within 1 minute at 37°C.
Enzyme activity is determined in the following mixture: 10 mM Tris-HCl (pH 7.5 at 25°C), 2.5 mM MgCl2, 0.1 mM CaCl2, and 1 µg of pUC19 DNA.
One unit of DNase I is equivalent to 0.3 Kunitz units.

Features
- Recombinant enzyme
- Purified from a non-animal host, with low levels of endogenous RNase

Scope of Application
- For the preparation of DNA-free RNA.
- To remove template DNA after in vitro transcription.
- To prepare DNA-free RNA before RT-PCR and RT-qPCR.
- In conjunction with DNA Polymerase I for DNA labeling through nick translation.
- For the study of DNA-protein interactions using DNase I (RNase-free) footprinting.
- To generate a library of randomly sheared DNA insert fragments. Reaction buffer containing Mn2+ is used.
Product tags

recombinant enzyme

,

application enzyme catalase

,

enzyme classification
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DNase I, RNase-Free Molecular Tool Enzyme

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Product Detail
Brand Name :
GDSBio
Model Number :
E1018
Certification :
/
Place of Origin :
China
MOQ :
1000 U
Price :
USD 76-84.5/1000 U
Contact Supplier
DNase I, RNase-Free Molecular Tool Enzyme

Guangzhou Dongsheng Biotech Co., Ltd

Active Member
4 Years
guangdong, guangzhou
Since 2005
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter
Main Products :
PCR Master Mix, Real Time PCR Mix, One Step RT QPCR Master Mix
Total Annual :
1000000-90000000
Employee Number :
50~100
Certification Level :
Active Member
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