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Molecular Biology Grade S1 Nuclease
Molecular Biology Grade S1 Nuclease
Specifications
Brand Name :
GDSBio
Model Number :
E1017
Certification :
/
Place of Origin :
China
MOQ :
10000 U
Price :
USD 58-64.5/10000 U
Payment Terms :
L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability :
100 Bag/Bags Per Day
Delivery Time :
8 work days
Packaging Details :
small package or bulk distribute or OEM
Product Name :
S1 Nuclease
Cat. No./Spec. :
E1017-A/10,000 U
Appearance :
colourless
Application :
degrade single-stranded nucleic acids
Classification :
General Reagents
Grade :
molecular biology
Logo Printing :
With Logo Printing
Transport Package :
Packing
Production Capacity :
100 Bag/Bags Per Day
Storage Conditions :
Store at -20°C
Description
S1 Nuclease

Cat. No./Spec.: E1017-A/10,000 U
Concentration: 100 U/µL

Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.
Storage Condition & Shelf Life
Store at -20°C.

Source
Aspergillus oryzae strain.

Unit Definition
A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C. Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl2, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.

Scope of Application
- Removal of single-stranded overhangs from DNA fragments
- Localization of S1 transcripts
- Cleavage of hairpin loops
- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments

Inhibition and Inactivation
- Inhibitors: metal chelating agents, PPi (inorganic pyrophosphate), Pi (inorganic phosphate), 5'-ribonucleotides, and
deoxyribonucleotides.
- Inactivate by heating at 70°C for 10 minutes in the presence of EDTA.
Product tags

charpy izod test

,

oligonucleotide calculator

,

charpy and izod test
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Molecular Biology Grade S1 Nuclease

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Product Detail
Brand Name :
GDSBio
Model Number :
E1017
Certification :
/
Place of Origin :
China
MOQ :
10000 U
Price :
USD 58-64.5/10000 U
Contact Supplier
Molecular Biology Grade S1 Nuclease

Guangzhou Dongsheng Biotech Co., Ltd

Active Member
4 Years
guangdong, guangzhou
Since 2005
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter
Main Products :
PCR Master Mix, Real Time PCR Mix, One Step RT QPCR Master Mix
Total Annual :
1000000-90000000
Employee Number :
50~100
Certification Level :
Active Member
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