【Intended Use】
This reagent kit is designed for efficient extraction of genomic DNA from Gram-negative bacteria and Gram-positive bacteria. It is suitable for use in both automated and semi-automated nucleic acid extraction workstations. The extracted DNA can be utilized in various downstream molecular biology experiments, including PCR, Southern blot analysis, fluorescence quantitative PCR, and other applications.
【Detection Principle】
DNA binds to the surface of Magbeads coated with silicon under high salt conditions. After multiple washes to remove impurities such as proteins, DNA is eluted under low salt conditions, resulting in high-purity genomic DNA.
【Main Components】
| Component | GP611-20 | GP611-50 | GP611-100 | GP611-300 |
| Suspension Buffer | 2.5mL | 6mL | 12.5mL | 37mL |
| Lysis Buffer | 9mL | 21mL | 45mL | 135mL |
| Wash Buffer | 6mL(+7.8mL Anhydrous ethanol) | 15mL(+19.5mL Anhydrous ethanol) | 30mL(+39mL Anhydrous ethanol) | 90mL(+117mL Anhydrous ethanol) |
| Elution Buffer | 3mL | 7mL | 15mL | 45mL |
| Magnetic Beads | 450μL | 1.05mL | 2*1.05mL | 6.5mL |
| Proteinase K | 450μL | 1.05mL | 2*1.05mL | 6.5mL |
| RNase A | 120μL | 300μL | 600μL | 1.8mL |
【Storage Conditions and Shelf Life】
Magbeads suspension should be stored at 2-8°C, while Proteinase K and RNase A should be stored below 4°C. The remaining reagents can be stored at room temperature, and the shelf life for all components is 1 year;
Transportation is permitted between 4-37°C, with a maximum transit time of 14 days.