【Intended Use】
This product is suitable for the extraction of large-volume plasmid DNA and can be subsequently utilized in various molecular experiments such as sequencing, enzymatic digestion, ligation, transformation, and other downstream molecular assays.
【Detection Principle】
DNA binds to the surface of Magbeads coated with silica under high-salt conditions. After multiple washes to remove impurities such as proteins, DNA is eluted under low-salt conditions, resulting in high-purity DNA.
【Main Components】
| Components | SP105-10 | SP105-20 | SP105-50 |
| Buffer1 | 32mL | 64mL | 160mL |
| Buffer2 | 32mL | 64mL | 160mL |
| Buffer3 | 32mL | 64mL | 160mL |
| Magnetic Beads | 1.1mL | 1.1mL*2 | 5.5mL |
| Washing Buffer | 32mL(+32mL Anhydrous ethanol) |
63mL(+63mL Anhydrous ethanol) |
158mL(+ 158mL Anhydrous ethanol) |
| Eluant | 11mL | 22mL | 55mL |
| RNase A | 0.32mL | 0.64mL | 1.6mL |
【Storage Conditions and Shelf Life】
The magnetic bead suspension should be stored at 2-8°C, RNase A below 4°C, and the remaining reagents at room temperature (15-25°C). All components have a shelf life of one year. Transportation is permissible between 4-37°C for up to 14 days.