96-Well Real-Time PCR System - 4/6-Channel Multiplex Q-PCR, 0.1 mL Tubes
The Accurate 96 is a 96-well real-time PCR system designed for quantitative nucleic acid analysis. It supports up to six fluorescence channels for multiplex qPCR, 10-50 μL reaction volumes and 0.1 mL PCR tubes or 96-well plates. The system features Peltier thermal cycling, PMT detection, a maximum heating and cooling rate of 6°C/s and a temperature range of 4-100°C.
System Overview
The Accurate 96 Real-Time PCR System combines thermal cycling and fluorescence detection for real-time monitoring of DNA amplification. The 96-well sample block accepts 0.1 mL PCR tubes and 96-well PCR plates, with a recommended reaction volume of 10-50 μL.
Key Features
- Peltier technology provides temperature range of 4-100°C
- Maximum heating and cooling rate of 6°C per second
- Temperature accuracy of ±0.2°C with uniformity of ±0.2°C at 60°C and 95°C
- Temperature gradient from 30 to 100°C supports PCR condition optimization
- Available with four or six fluorescence channels supporting multiplex qPCR
- High-efficiency LED excitation with PMT detection
- Time-resolved signal separation reduces fluorescence crosstalk
- Edge temperature compensation minimizes well-to-well differences
- Detection sensitivity of single-copy gene with dynamic range up to 10 orders of magnitude
Applications
- Quantitative gene expression analysis
- Multiplex qPCR assays
- DNA and RNA target detection
- Pathogen research and laboratory testing
- Genotyping and mutation analysis
- Food safety and GMO testing
- Agricultural and veterinary research
- Environmental microbiology
- Forensic and academic research workflows
Technical Specifications
| Specification |
Accurate 96 |
| Sample Capacity |
96 |
| Reaction Volume |
10-50 μl |
| Thermal Cycle Technology |
Peltier |
| Max. Heating/Cooling Rate |
6.0°C/s |
| Heating Temperature Range |
4-100°C |
| Lid Temperature Range |
30-115°C |
| Temperature Control Accuracy [at 55°C] |
±0.2°C |
| Temperature Uniformity [at 35-72°C] |
±0.2°C @60°C, ±0.3°C @95°C |
| Gradient Temperature Setting Range |
30-100°C |
| Gradient Range |
1-36°C |
| Detection System |
| Excitation Light Source |
4/6 monochrome high efficiency LEDs |
| Detection Device |
PMT |
| Detection Mode |
Time-resolved signal separating technology |
| Excitation/Detection Wavelength Range |
455-650nm/510-715nm |
| Fluorescent Channels |
4/6 channels |
| Supported Dye |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity |
Single copy gene |
| Resolution |
1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic Range |
10 orders of magnitude copies |
Note: Actual performance depends on selected reagents, assay design, sample quality and operating conditions. Use for clinical diagnosis requires applicable local registration, validated assays and regulatory approval.