The Nano-500 combines a full-spectrum microvolume UV-Vis spectrophotometer, a fluorescence detector and an OD600 cuvette reader in one compact instrument.
Key Specifications:
The Nano-500 Microvolume Spectrophotometer and Fluorometer is designed for both high-concentration absorbance measurement and low-concentration fluorescence quantification.
The absorbance system uses a xenon flash lamp and a 2048-element linear CCD detector to measure samples across 200-800 nm. Three optical path lengths—0.05, 0.2 and 1.0 mm—allow direct analysis of a wide concentration range. The instrument measures dsDNA from 2 to 15,000 ng/μL and BSA protein from 0.1 to 450 mg/mL.
Only 0.5-2.0 μL of sample is required. The motor-driven sample stretching system helps create a stable liquid column and improve repeatability, particularly for concentrated or viscous samples. Results are displayed in less than 6 seconds.
Fluorescence mode uses LED excitation and photodiode detection. With a compatible fluorescence quantification kit, it can measure low-concentration DNA, RNA or protein samples. The fluorescence measurement time is approximately 3 seconds.
Optional Configurations:
An independent OD600 cuvette mode is provided for bacterial and microbial culture measurement.
The 7-inch touchscreen and Android system allow standalone operation. Results can be transferred through USB or printed directly using the built-in printer.
Fluorescence performance depends on the installed channel, compatible reagent kit and assay conditions. UV, green and red fluorescence channels are optional configurations and are not included simultaneously in the standard blue-channel model.
| Parameter | Nano-500 |
|---|---|
| Product Type | Microvolume Spectrophotometer and Fluorometer |
| Absorbance Wavelength Range | 200-800 nm |
| Sample Volume | 0.5-2.0 μL |
| Optical Path Length | 0.05, 0.2 and 1.0 mm |
| Light Source | Xenon flash lamp |
| Detector | 2048-element linear CCD array |
| Wavelength Accuracy | 1 nm |
| Spectral Resolution | ≤3 nm |
| Absorbance Precision | 0.003 Abs |
| Absorbance Accuracy | 1% at 7.332 Abs, 260 nm |
| Absorbance Range | 0.04-300 A |
| dsDNA Detection Range | 2-15,000 ng/μL |
| BSA Protein Detection Range | 0.1-450 mg/mL |
| Measurement Time | <6 seconds |
| Sample Pedestal Material | Aluminum alloy and quartz fiber |
| Operating System | Android |
| Display | 7-inch capacitive touchscreen |
| Data Transfer | USB |
| Printer | Built-in printer |
| Dimensions (W × D × H) | 208 × 320 × 186 mm |
| Weight | 3.6 kg |
| Operating Voltage | DC 24 V, 2 A |
| Operating Power | 25 W |
| Standby Power | 5 W |
| Parameter | Nano-500 |
|---|---|
| Fluorescence Light Source | LED |
| Fluorescence Detector | Photodiode |
| Sensitivity | 0.5 pg/μL dsDNA under optimized conditions |
| Dynamic Range | 5 orders of magnitude |
| Linearity | R² ≥0.995 |
| Repeatability | ≤1.5% |
| Stability | ≤1.5% |
| Measurement Time | Approximately 3 seconds |
| Standard Fluorescence Channel | Blue |
| Parameter | Nano-500 |
|---|---|
| Measurement Mode | OD600 cuvette measurement |
| Light Source | LED |
| Wavelength | 600 ±8 nm |
| Absorbance Range | 0-4 A |
| Applications | Bacterial and microbial culture concentration measurement |