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Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents
Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents
Specifications
Brand Name :
Amp-future bio
Model Number :
WLRN8211KIT
Certification :
ISO9001
Place of Origin :
China
MOQ :
48T
Price :
USD3.8$/T
Payment Terms :
T/T paypal
Supply Ability :
100000T/Month
Delivery Time :
6days
Packaging Details :
16T/Bag,48T/Box
Application :
RNA Nucleic Acid Amplification
Components :
Primers, Taq DNA Polymerase, dNTPs, Mg2+
Detection Method :
NFO
Instrument :
Test strips
Kit Size :
48 reactions
Product Format :
Lyophilized
Product Name :
RNA Isothermal Amplification NFO Kits
Product Type :
Kit
Reaction Time :
5-20 minutes
Reaction Volume :
50μl
Shelf Life :
14 months
Shipping Condition :
Ice
Storage Temperature :
-20°C
Target Template :
RNA
Description

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3' end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:
Parameters Details
Product Name RNA Isothermal Amplification Kit NFO
Manufacturer Amp-future
Storage Temperature -20°C
Kit Components Enzymes, Buffers ,Reagents
Packaging 48 Tests/box
Detection Limit 500-1000copies/µL
Shipping ICE
Test Time 5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents

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Product Detail
Brand Name :
Amp-future bio
Model Number :
WLRN8211KIT
Certification :
ISO9001
Place of Origin :
China
MOQ :
48T
Price :
USD3.8$/T
Contact Supplier
Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents

Amp-Future (Changzhou) Biotech Co., Ltd.

Active Member
2 Years
jiangsu, changzhou
Since 2021
Business Type :
Manufacturer, Importer, Exporter
Main Products :
Isothermal Amplification Kit, DNA Amplification Kit, RNA Amplification Kit
Total Annual :
100000-500000
Employee Number :
50~80
Certification Level :
Active Member
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