Specifications
Brand Name :
REAGEN
Model Number :
RNC95009
Place of Origin :
USA
MOQ :
5 kits
Price :
Negotiable
Payment Terms :
T/T
Supply Ability :
100 kits per month
Delivery Time :
5-7 days
Packaging Details :
color packing
Type :
reagent
Size :
96 test
Package :
color packing
Shelf life :
one year
Sensitivity :
0.8ppb
Description

Fructose/Glucose Assay Kit

Product Description

REAGEN Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples.

The unique features of the kit are:

1.High sensitivity and low detection limit (0.8 ppm)

2.High reproducibility

3.A rapid (30 minute), robust enzyme-based assay

Procedure Overview

REAGEN Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.

Kit Contents, Storage and Shelf Life

REAGEN Fructose/Glucose Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.

Kit Contents Amount Storage
Dilution Buffer 10 mL 4ºC
Substrate Mix 20 mL 4ºC
Hexokinase 0.7 mL 4ºC
PGI Enzyme 0.2 mL 4ºC
Microtiter Plate One 4ºC

Sensitivity (Detection Limit)

Sample Volume Detection Limit (mg/L)
0.01 mL 8
0.1mL 0.8

Fructose / Glucose Assay Kit Food Composition Testing Robust Enzyme Based Assay

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Fructose / Glucose Assay Kit Food Composition Testing Robust Enzyme Based Assay

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Brand Name :
REAGEN
Model Number :
RNC95009
Place of Origin :
USA
MOQ :
5 kits
Price :
Negotiable
Payment Terms :
T/T
Contact Supplier
Fructose / Glucose Assay Kit Food Composition Testing Robust Enzyme Based Assay

REAGEN LLC

Verified Supplier
6 Years
Since 2012
Business Type :
Manufacturer
Total Annual :
15000000-20000000
Employee Number :
50~80
Certification Level :
Verified Supplier
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