This kit choose Canines/dogs' Toxplasma conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of canines/dogs' Toxplasma was detected by fluorescence PCR in a completely closed reaction system.
Component
Specification
PCR reaction liquid
8 tubes
Positive control
1 tube
Negative control
1 tube
Storage: keep away from direct light uner -20±5℃
The results of Analysis
Results
Ct Value
Positive
Ct≤40
Negative
No Ct value or Ct value=0
Gray area
40<Ct≤45
Operation details please check from IFU!
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Canine Toxoplasma Test Kit CTOX Polymerase Dog DNA QPCR Taqman Probe