Testing Principle
This method mainly uses acid fuchsin and picric acid to double dye the collagen fiber and the muscle fiber respectively. The bitter acid has a small molecular weight, can penetrate muscle fibers, glial cells and red blood cells with dense tissue structure combining with bitter acid were stained in yellow; The acid fuchsin has a medium to large molecular weight, and can only penetrate collagen fibers with looser tissue structure. combining with acid fuchsin were stained in to red to pink.
Stain Results
| Collagen fibers | Red |
| Muscle fibers, Nerve glial, Erythrocytes | Yellow |
| Nuclei | Purple black |

Packaging Specification
| Product Name | Ref # | Specification |
| Van Gieson Stain | BA4084A | 3x20ml |
| Van Gieson Stain | BA4084B | 3x100ml |