Specifications
Brand Name :
SHIHONG
Model Number :
PEPTONE
Certification :
SGS
Place of Origin :
CHINA
MOQ :
1MT
Price :
USD 2.3/KG - USD 3.1$/KG
Payment Terms :
L/C, D/A, D/P, T/T
Supply Ability :
2,000MT/MONTH
Delivery Time :
5 WORKING DAYS
Packaging Details :
20KG/BAG 25KG/BAG
Description

Biological Grade Bone Peptone — High-Purity Nitrogen Substrate for Microbial Fermentation

Product Summary

Biological Grade Bone Peptone is a specialized nitrogen substrate derived from fresh bovine bone through a controlled enzymatic digestion process. This product serves as an essential nutrient base for industrial fermentation, microbiological culture media preparation, and biopharmaceutical applications, providing readily assimilable nitrogen sources in a highly soluble, low-residue format.


Production Methodology

Processing Phase Operating Parameters
Raw Material Pre-treatment Fresh bovine bone selection, thermal sanitation, particulate reduction
Enzymatic Digestion Pancreatic enzyme hydrolysis under controlled temperature conditions
Primary Separation Fractionation and initial clarification
Purification Sequence Chromophore removal, odor reduction, membrane filtration
Concentration Pathway Ultrafiltration, thermal concentration, sterile filtration
Final Processing Atomization drying, particle classification, quality verification

Functional Characteristics

The product exhibits the following functional attributes that support consistent microbial cultivation:

Characteristic Description
Nitrogen Availability Dual nitrogen fractions (total and amino) support both primary metabolism and specific biosynthetic pathways
Solution Behavior Complete solubility with thermal stability; no precipitation under standard sterilization conditions
Mineral Profile Controlled sodium chloride and ash content minimize osmotic and inhibitory effects
Biological Compatibility Supports growth of fastidious organisms without supplementation

Technical Specifications

Parameter Unit Certified Limit Analytical Result
Total Nitrogen Fraction % (w/w) ≥ 14.5 15.2
Amino Nitrogen Fraction % (w/w) ≥ 2.5 3.1
Sodium Chloride Content % (w/w) ≤ 5.0 2.56
Residual Moisture % (w/w) ≤ 5.0 4.5
Mineral Residue (Ash) % (w/w) ≤ 5.0 3.62
Hydrogen Ion Concentration (2% solution) pH units 5.0 – 7.0 5.4
Visual Appearance Yellow granular powder Conforms
Aqueous Clarity (2%) Clear solution, no sediment Conforms
Alkaline Stability (pH 8-9, 121°C) No precipitation Conforms

Strain-Based Performance Validation

Assay Type Test Microorganism Expected Reaction Observed Performance
Sulfhydryl Amino Acid Detection Salmonella typhi / Shigella Positive / Negative Meets specifications
Tryptophan Metabolism E. coli / S. typhi Positive / Negative Meets specifications
Glucose Metabolic Pathway (V-P) Enterobacter aerogenes / E. coli Positive / Negative Meets specifications
Carbohydrate Utilization E. coli / S. typhi No acid formation Meets specifications
Erythrocyte Interaction S. pneumoniae / β-streptococcus Alpha / Beta patterns Maintained through passages
Chromophore Expression P. aeruginosa / S. aureus Green / Gold pigmentation Maintained through passages
Colony Development E. coli / S. aureus Diameter ≥1.0mm, smooth morphology Meets specifications
Minimum Inoculum Response Streptococcus / Gram-positive bacillus Growth at 10⁻⁴ / 10⁻⁵ dilution Meets specifications

Application Areas

Sector Specific Applications
Industrial Biotechnology Microbial enzyme production, organic acid fermentation, single-cell protein cultivation
Diagnostic Manufacturing Dehydrated culture media, selective media formulations, transport media
Pharmaceutical Production Bacterial vaccine manufacturing, toxoid production, fermentor seed cultures
Research Institutions Microbial physiology studies, strain development, media optimization

Physical Form and Storage

Attribute Specification
Packaging Configuration 20 kg units with moisture-protective inner liner and durable outer container
Storage Parameters Temperature-controlled environment (≤25°C), relative humidity ≤60%
Shelf Stability 24 months from manufacture date under recommended conditions
Material Handling Minimize atmospheric exposure; reseal containers promptly after use

Quality Framework

  • Internal Standard: Q/SCSH 001-2025

  • Reference Document: Chinese Biological Products Main Raw and Auxiliary Materials Quality Control Standard (2000 Edition)

  • Analytical Methods: GB 5009 series (moisture, ash, nitrogen, pH, sodium chloride) and pharmacopoeial references


Values shown represent lot analysis. Performance characteristics may vary slightly between production batches. Technical consultation available for specialized application requirements.

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Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation

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Brand Name :
SHIHONG
Model Number :
PEPTONE
Certification :
SGS
Place of Origin :
CHINA
MOQ :
1MT
Price :
USD 2.3/KG - USD 3.1$/KG
Contact Supplier
video
Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation
Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation
Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation
Biological Grade Bone Peptone - High-Purity Nitrogen Substrate for Microbial Fermentation

Sichuan Shihong Technology Co.,Ltd

Verified Supplier
5 Years
sichuan, chengdu
Since 2017
Business Type :
Manufacturer, Exporter
Total Annual :
5 million-1 billion
Employee Number :
180~200
Certification Level :
Verified Supplier
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